Quantitation of digitoxin, digoxin, and their metabolites by high-performance liquid chromatography using pulsed amperometric detection
نویسندگان
چکیده
A novel detection method for the cardiac glycosides digitoxin, digoxin, and their metabolites is described. Both isocratic and gradient water-acetonitrile elutions were employed with an octadecylsilane column. The effluent was monitored by integrated pulsed amperometry in an alkaline medium (pH> 12) by adding sodium hydroxide post-column. Using isocratic elution, peak responses were linear from 10 to 70 ng, with lower limits of detection between 4 and 15 ng. This method allows easy modification of well-established high-performance liquid chromatographic systems, as well as comparable sensitivity and greater selectivity than UV detection.
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